[In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 40(4): 319-22, 2005 Jul.
Article
in Zh
| MEDLINE
| ID: mdl-16191378
ABSTRACT
OBJECTIVE:
To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.METHODS:
Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.RESULTS:
Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.CONCLUSIONS:
On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Cranial Nerves
/
Fibroblast Growth Factor 8
/
Mesoderm
/
Neural Crest
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Kou Qiang Yi Xue Za Zhi
Journal subject:
ODONTOLOGIA
Year:
2005
Document type:
Article
Affiliation country:
China