[Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome].
Beijing Da Xue Xue Bao Yi Xue Ban
; 37(5): 476-9, 2005 Oct 18.
Article
in Zh
| MEDLINE
| ID: mdl-16224516
ABSTRACT
OBJECTIVE:
To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome (PCOS) and normal ovary, and explore the pathogenesis of PCOS.METHODS:
The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining.RESULTS:
The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary (P<0.05). PDCD5-positive cells were mainly observed in cytoplasm. There was no significant difference between the two groups in terms of age, basal serum FSH concentration, number of ampules of FSH administered.CONCLUSION:
Our data suggest that PDCD5 is an important apoptosis regulating factor in granulosa cells of PCOS and normal ovary, which might be involved in the pathogenesis of PCOS.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polycystic Ovary Syndrome
/
Apoptosis Regulatory Proteins
/
Granulosa Cells
/
Neoplasm Proteins
Limits:
Adult
/
Female
/
Humans
Language:
Zh
Journal:
Beijing Da Xue Xue Bao Yi Xue Ban
Journal subject:
MEDICINA
Year:
2005
Document type:
Article
Affiliation country:
China