The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation.
Biochem Biophys Res Commun
; 339(3): 905-14, 2006 Jan 20.
Article
in En
| MEDLINE
| ID: mdl-16329992
ABSTRACT
The Tup1-Ssn6 corepressor regulates the expression of diverse classes of genes in Saccharomyces cerevisiae. Chromatin is an important component of Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated tails of histones H3 and H4, and requires multiple histone deacetylases for the repression. Here we examine if histone methylation, in addition to histone deacetylation, plays a role in Tup1-Ssn6 repression. We found that like other genes, Tup1-Ssn6 target genes exhibit increased levels of histone H3 lysine 4 trimethylation upon activation. However, deletion of individual or multiple histone methyltransferases and other SET-domain containing genes has no apparent effect on Tup1-Ssn6-mediated repression of a number of well-defined targets. Interestingly, we discovered that Ssn6 interacts with Set2. Although deletion of SET2 does not affect Tup1-Ssn6 repression of a number of target genes, Ssn6 may utilize Set2 in specific contexts to regulate gene repression.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Saccharomyces cerevisiae
/
Nuclear Proteins
/
Histones
/
Signal Transduction
/
Saccharomyces cerevisiae Proteins
/
DNA-Binding Proteins
/
Methyltransferases
Type of study:
Risk_factors_studies
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2006
Document type:
Article
Affiliation country:
Estados Unidos