Effects of exogenous p53 transfection on the gene expression in the human brain glioma cell line U251.
Colloids Surf B Biointerfaces
; 47(2): 126-31, 2006 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-16413759
ABSTRACT
The p53 gene is activated in response to several malignancy-associated stress signals by transactivation of downstream genes and by transcription-independent mechanisms. In order to identify new p53 downstream genes, we established a new system of p53 gene inducible expression, U251-pTet-p53 cell line, with the Tet-On Gene Expression System, in which exogenous p53 gene could overexpress in doxycycline (Dox) medium but not in the medium without Dox. By comparing their random primer RT-PCR products, it was proved that exogenous p53 gene expression could lead to many genes differential expression, some up-expressed and others down-expressed. All of these differential expressed genes may be p53 downstream genes. We can gain the magnitude of p53 downstream genes, which provides the basis of directly cloning of novel p53 downstream genes and further studying of p53 regulatory network.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Neoplasms
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Tumor Suppressor Protein p53
/
Cell Line, Tumor
/
Glioma
Limits:
Humans
Language:
En
Journal:
Colloids Surf B Biointerfaces
Journal subject:
QUIMICA
Year:
2006
Document type:
Article
Affiliation country:
China