Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi.
Plant Cell Rep
; 25(6): 561-72, 2006 Jun.
Article
in En
| MEDLINE
| ID: mdl-16456649
ABSTRACT
Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. Beta-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of beta-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic beta-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing beta-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rhizoctonia
/
Arabidopsis
/
Plant Leaves
/
Antimicrobial Cationic Peptides
/
Pseudomonas syringae
/
Fusarium
Language:
En
Journal:
Plant Cell Rep
Journal subject:
BOTANICA
Year:
2006
Document type:
Article
Affiliation country:
Estados Unidos