Epitope enhancement of a CD4 HIV epitope toward the development of the next generation HIV vaccine.
J Immunol
; 176(6): 3753-9, 2006 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-16517744
ABSTRACT
Virus-specific CD4+ T cell help and CD8+ cytotoxic T cell responses are critical for maintenance of effective immunity in chronic viral infections. The importance of CD4+ T cells has been documented in HIV infection. To investigate whether a stronger CD4+ T cell response can be induced by modifications to enhance the T1 epitope, the first CD4+ T cell epitope discovered in HIV-1-gp120, we developed a T1-specific CD4+ T cell line from a healthy volunteer immunized with a canarypox vector expressing gp120 and boosted with recombinant gp120. This T1-specific CD4+ T cell line was restricted to DR13, which is common in U.S. Caucasians and African-Americans and very frequent in Africans. Peptides with certain amino acid substitutions in key positions induced enhanced specific CD4+ T cell proliferative responses at lower peptide concentration than the original epitope. This relatively conserved CD4 epitope improved by the epitope enhancement strategy could be a component of a more effective second generation vaccine construct for HIV infection.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
CD4-Positive T-Lymphocytes
/
HIV-1
/
AIDS Vaccines
/
Epitopes, T-Lymphocyte
Limits:
Humans
Language:
En
Journal:
J Immunol
Year:
2006
Document type:
Article
Affiliation country:
Estados Unidos