Leishmania donovani requires functional Cdc42 and Rac1 to prevent phagosomal maturation.
Infect Immun
; 74(5): 2613-8, 2006 May.
Article
in En
| MEDLINE
| ID: mdl-16622197
ABSTRACT
Leishmania donovani promastigotes survive inside macrophage phagosomes by inhibiting phagosomal maturation. The main surface glycoconjugate on promastigotes, lipophosphoglycan (LPG), is crucial for survival and mediates the formation of a protective shell of F-actin around the phagosome. Previous studies have demonstrated that this effect involves inhibition of protein kinase C alpha. The present study shows that functional Cdc42 and Rac1 are required for the formation of F-actin around L. donovani phagosomes. Moreover, we present data showing that phagosomes containing LPG-defective L. donovani, which is unable to induce F-actin accumulation, display both elevated levels of periphagosomal F-actin and impaired phagosomal maturation in macrophages with permanently active forms of Cdc42 and Rac1. We conclude that L. donovani engages Cdc42 and Rac1 to build up a protective coat of F-actin around its phagosome to prevent phagosomal maturation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leishmania donovani
/
Phagosomes
/
Cdc42 GTP-Binding Protein
/
Rac1 GTP-Binding Protein
Limits:
Animals
Language:
En
Journal:
Infect Immun
Year:
2006
Document type:
Article
Affiliation country:
Suecia