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Asynchronous expression of myeloid antigens in leukemic cells in a PML/RARalpha transgenic mouse model.
Santana, B A A; Pintão, M C; Abreu e Lima, R S; Scheucher, P S; Santos, G A S; Garcia, A B; Falcão, R P; Rego, E M.
Affiliation
  • Santana BA; Divisão de Hematologia e Centro de Terapia Celular, Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Braz J Med Biol Res ; 39(5): 615-20, 2006 May.
Article in En | MEDLINE | ID: mdl-16648899
Acute promyelocytic leukemia (APL) is characterized by the expansion of blasts that resemble morphologically promyelocytes and harbor a chromosomal translocation involving the retinoic acid receptor alpha (RARalpha) and the promyelocytic leukemia (PML) genes on chromosomes 17 and 15, respectively. The expression of the PML/RARalpha fusion gene is essential for APL genesis. In fact, transgenic mice (TM) expressing PML/RARalpha develop a form of leukemia that mimics the hematological findings of human APL. Leukemia is diagnosed after a long latency (approximately 12 months) during which no hematological abnormality is detected in peripheral blood (pre-leukemic phase). In humans, immunophenotypic analysis of APL blasts revealed distinct features; however, the precise immunophenotype of leukemic cells in the TM model has not been established. Our aim was to characterize the expression of myeloid antigens by leukemic cells from hCG-PML/RARalpha TM. In this study, TM (N = 12) developed leukemia at the mean age of 13.1 months. Morphological analysis of bone marrow revealed an increase of the percentage of immature myeloid cells in leukemic TM compared to pre-leukemic TM and wild-type controls (48.63 +/- 16.68, 10.83 +/- 8.11, 7.4 +/- 5.46%, respectively; P < 0.05). Flow cytometry analysis of bone marrow and spleen from leukemic TM identified the asynchronous co-expression of CD34, CD117, and CD11b. This abnormal phenotype was rarely detected prior to the diagnosis of leukemia and was present at similar frequencies in hematologically normal TM and wild-type controls of different ages. The present results demonstrate that, similarly to human APL, leukemic cells from hCG-PML/RARalpha TM present a specific immunophenotype.
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Collection: 01-internacional Database: MEDLINE Main subject: Leukemia, Myeloid, Acute / Leukemia, Promyelocytic, Acute / Antigens, CD / Oncogene Proteins, Fusion Type of study: Prognostic_studies Limits: Animals Language: En Journal: Braz J Med Biol Res Year: 2006 Document type: Article Affiliation country: Brasil Country of publication: Brasil
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Collection: 01-internacional Database: MEDLINE Main subject: Leukemia, Myeloid, Acute / Leukemia, Promyelocytic, Acute / Antigens, CD / Oncogene Proteins, Fusion Type of study: Prognostic_studies Limits: Animals Language: En Journal: Braz J Med Biol Res Year: 2006 Document type: Article Affiliation country: Brasil Country of publication: Brasil