Resistin-like molecule beta regulates innate colonic function: barrier integrity and inflammation susceptibility.
J Allergy Clin Immunol
; 118(1): 257-68, 2006 Jul.
Article
in En
| MEDLINE
| ID: mdl-16815164
ABSTRACT
BACKGROUND:
Resistin-like molecule (RELM) beta is a cysteine-rich cytokine expressed in the gastrointestinal tract and implicated in insulin resistance and gastrointestinal nematode immunity; however, its function primarily remains an enigma.OBJECTIVE:
We sought to elucidate the function of RELM-beta in the gastrointestinal tract.METHODS:
We generated RELM-beta gene-targeted mice and examined colonic epithelial barrier function, gene expression profiles, and susceptibility to acute colonic inflammation.RESULTS:
We show that RELM-beta is constitutively expressed in the colon by goblet cells and enterocytes and has a role in homeostasis, as assessed by alterations in colon mRNA transcripts and epithelial barrier function in the absence of RELM-beta. Using acute colonic inflammatory models, we demonstrate that RELM-beta has a central role in the regulation of susceptibility to colonic inflammation. Mechanistic studies identify that RELM-beta regulates expression of type III regenerating gene (REG) (REG3beta and gamma), molecules known to influence nuclear factor kappaB signaling.CONCLUSIONS:
These data define a critical role for RELM-beta in the maintenance of colonic barrier function and gastrointestinal innate immunity. CLINICAL IMPLICATIONS These findings identify RELM-beta as an important molecule in homeostatic gastrointestinal function and colonic inflammation, and as such, these results have implications for a variety of human inflammatory gastrointestinal conditions, including allergic gastroenteropathies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Colitis
/
Colon
/
Hormones, Ectopic
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Allergy Clin Immunol
Year:
2006
Document type:
Article
Affiliation country:
Estados Unidos