Dendritic cell surface calreticulin is a receptor for NY-ESO-1: direct interactions between tumor-associated antigen and the innate immune system.
J Immunol
; 177(6): 3582-9, 2006 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-16951317
ABSTRACT
How the immune system recognizes endogenously arising tumors and elicits adaptive immune responses against nonmutated tumor-associated Ags is poorly understood. In search of intrinsic factors contributing to the immunogenicity of the tumor-associated Ag NY-ESO-1, we found that the NY-ESO-1 protein binds to the surface of immature dendritic cells (DC), macrophages, and monocytes, but not to that of B cells or T cells. Using immunoprecipitation coupled with tandem mass spectrometry, we isolated DC surface calreticulin as the receptor for NY-ESO-1. Calreticulin Abs blocked NY-ESO-1 binding on immature DC and its cross-presentation to CD8+ T cells in vitro. Calreticulin/NY-ESO-1 interactions provide a direct link between NY-ESO-1, the innate immune system, and, potentially, the adaptive immune response against NY-ESO-1.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dendritic Cells
/
Receptors, Cell Surface
/
Calreticulin
/
Immunity, Innate
/
Membrane Proteins
/
Antigens, Neoplasm
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
J Immunol
Year:
2006
Document type:
Article
Affiliation country:
Estados Unidos