Mouse microRNA profiles determined with a new and sensitive cloning method.
Nucleic Acids Res
; 34(17): e115, 2006.
Article
in En
| MEDLINE
| ID: mdl-16973894
ABSTRACT
MicroRNAs (miRNAs) are noncoding RNA molecules of 21 to 24 nt that regulate the expression of target genes in a post-transcriptional manner. Although evidence indicates that miRNAs play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases, extensive miRNA profiling in cells or tissues has been hampered by the lack of sensitive cloning methods. Here we describe a highly efficient profiling method, termed miRNA amplification profiling (mRAP), as well as its application both to mouse embryos at various developmental stages and to adult mouse organs. A total of 77,436 Small-RNA species was sequenced, with 11,776 of these sequences found to match previously described miRNAs. With the use of a newly developed computational prediction algorithm, we further identified 229 independent candidates for previously unknown miRNAs. The expression of some of these candidate miRNAs was confirmed by northern blot analysis and whole-mount in situ hybridization. Our data thus indicate that the total number of miRNAs in vertebrates is larger than previously appreciated and that the expression of these molecules is tightly controlled in a tissue- and developmental stage-specific manner.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cloning, Molecular
/
Gene Expression Profiling
/
MicroRNAs
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Nucleic Acids Res
Year:
2006
Document type:
Article
Affiliation country:
Japón