Effects of cytoskeletal perturbation on the sensitivity of Ehrlich ascites tumor cell surface membranes to mechanical trauma.
Invasion Metastasis
; 11(2): 93-101, 1991.
Article
in En
| MEDLINE
| ID: mdl-1717396
ABSTRACT
Evidence presented previously indicated that shape changes in circulating cancer cells within the microvasculature may lead to rapid, lethal rupture of the cell surface membranes, thereby contributing to the inefficiency of this phase of hematogenous metastasis. As the cytoskeleton is known to regulate cell shape and, in at least some cases, to be attached to the surface membrane, we have determined whether or not it plays a role in inhibiting lethal, deformation-associated, mechanically induced surface membrane trauma. Thus, Ehrlich ascites tumor (EAT) cells were treated with different cytoskeleton-perturbing agents, and their susceptibility to filtration trauma on passage through Nuclepore membranes was determined. In contrast to the involvement of the cytoskeleton in nonlethal cell deformation, agents which disrupt intracellular networks of microtubules, microfilaments and intermediate filaments had little or no direct effect on EAT cell susceptibility to rapid, mechanically induced, lethal trauma.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cytoskeleton
/
Carcinoma, Ehrlich Tumor
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Invasion Metastasis
Year:
1991
Document type:
Article