Homologous regulation of estrogen receptor subtypes in goldfish (Carassius auratus).
Mol Reprod Dev
; 74(9): 1105-12, 2007 Sep.
Article
in En
| MEDLINE
| ID: mdl-17342729
ABSTRACT
While considerable information is available on the physiological effects of estrogen, much less is known about the regulation of estrogen receptor (ER) subtypes, particularly in non-mammalian vertebrates. Using goldfish as primary experimental model, we investigated sex- and tissue-specific homologous regulation of ER subtypes (ERalpha, ERbetaI, and ERbetaII) by estradiol in vivo, in the liver and gonads. Treatment with estradiol, significantly upregulated transcript levels for all three types of ERs (ERalpha, ERbetaI, and ERbetaII) in the goldfish ovary and testis. In the goldfish liver, treatment with estradiol significantly increased ERalpha, ERbetaI transcript levels without affecting ERbetaII. In all cases increased ER transcript level was correlated with increased ER protein level determined by Western blot analysis, although we are not able to distinguish between ER subtypes. The results provide strong support for the hypothesis that homologous regulation of ERs is tissue- and gender-specific, and may be a mechanism for estrogen-mediated regulation of reproduction in goldfish.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reproduction
/
Goldfish
/
Receptors, Estrogen
/
Estradiol
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Reprod Dev
Journal subject:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Year:
2007
Document type:
Article
Affiliation country:
Canadá