Nitric oxide increases in the rat retina after continuous illumination.
Brain Res
; 1156: 112-9, 2007 Jul 02.
Article
in En
| MEDLINE
| ID: mdl-17499222
ABSTRACT
Continuous illumination (CI) of the retina induces an oxidative stress followed by the degeneration of photoreceptors. This phenomenon may be partially related to the excessive production of nitric oxide (NO). In order to confirm this hypothesis, the aims of this work are to determine NO levels during the illumination of the retina by electron paramagnetic resonance (EPR), and if an increase of NO is found, to characterize the NOS isoform responsible of the increment by using Western blot. Sprague-Dawley rats were continuously illuminated with white light (12,000 lux) for 2, 24, 48 h, 5 and 7 days while control rats were maintained at light/dark cycles of 12/12 h. Using EPR, an increase of NO signal was observed in the light exposed retinas peaking at 24 h of CI. Western blot analysis showed the expression of iNOS in the illuminated retinas with a peak after 24 h of CI, but did not show significant differences of nNOS among illuminated and control retinas. In summary, there is an increase of NO during CI. Further studies will reveal whether this mechanism is responsible for light induced photoreceptor degeneration.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retina
/
Nitric Oxide
Limits:
Animals
Language:
En
Journal:
Brain Res
Year:
2007
Document type:
Article
Affiliation country:
Argentina