Reduced adiposity in ob/ob mice following total body irradiation and bone marrow transplantation.
Obesity (Silver Spring)
; 15(6): 1419-29, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17557979
ABSTRACT
OBJECTIVE:
The objective of this study was to assess long-term metabolic consequences of total body irradiation (TBI) and bone marrow transplantation. Severe obesity develops due to both hypertrophy and hyperplasia of adipocytes. We hypothesized that TBI would arrest adipose tissue growth and would affect insulin resistance (IR). RESEARCH METHODS AND PROCEDURES We exposed 2-month-old female ob/ob mice to 8 Grays of TBI followed by bone marrow transplantation and tested the animals for body weight (BW) gain, body composition, blood glucose, and insulin sensitivity.RESULTS:
Two months after TBI, irradiated mice stopped gaining BW, whereas non-treated mice continued to grow. At the age of 9.5 months, body mass of irradiated mice was 60.6 +/- 1.4 grams, which was only 61% of that in non-treated ob/ob controls (99.4 +/- 1.6 grams). Body composition measurements by DXA showed that decreased BW was primarily due to an impaired fat accumulation. This could not result from the production of leptin by bone marrow-derived adipocyte progenitors because inhibition of the obese phenotype was identical in recipients of both B6 and ob/ob bone marrow. Inability of the irradiated mice to accumulate fat was associated with hepatomegaly, lower levels of monocyte chemoattractant protein-1 expression in adipose tissue, and increased IR.DISCUSSION:
Our data argue in favor of the hypothesis that inability of adipose tissue to expand may increase IR. This mouse model may be valuable for studies of late-onset radiation-induced IR in humans.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Whole-Body Irradiation
/
Bone Marrow Transplantation
/
Adiposity
/
Obesity
Type of study:
Etiology_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Obesity (Silver Spring)
Journal subject:
CIENCIAS DA NUTRICAO
/
FISIOLOGIA
/
METABOLISMO
Year:
2007
Document type:
Article
Affiliation country:
Estados Unidos