Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata.
Pol J Microbiol
; 56(2): 139-47, 2007.
Article
in En
| MEDLINE
| ID: mdl-17650684
ABSTRACT
A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing on Polybuffer Exchanger PBE-94.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Basidiomycota
/
Beta-Mannosidase
Language:
En
Journal:
Pol J Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2007
Document type:
Article
Affiliation country:
Polonia