Non-specific antiviral response detected in RNA-treated cultured cells of the sandfly, Lutzomyia longipalpis.
Dev Comp Immunol
; 32(3): 191-7, 2008.
Article
in En
| MEDLINE
| ID: mdl-17706772
ABSTRACT
Lutzomyia longipalpis is the principal vector of visceral leishmaniasis in the Americas, and can also transmit some viruses. To help develop a gene-silencing system for this sandfly, we transfected cultured embryonic cells with various double-stranded RNAs using West Nile virus (WNV) virus-like particles (VLPs) expressing luciferase as the target RNA to demonstrate effective gene knock-down. When luciferase dsRNA was introduced into these cells, they produced the expected reduction in VLP-encoded luciferase, suggesting specific silencing of the luciferase gene. Surprisingly, we found that unrelated dsRNAs, which included those specific for several L. longipalpis gene sequences and Escherichia coli beta-galactosidase, diminished replication of the VLP-encoded genome. These results are the first indication for a nucleic acid-induced, non-specific antiviral response in this important insect vector.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psychodidae
/
West Nile virus
/
RNA, Double-Stranded
/
Luciferases
Limits:
Animals
Language:
En
Journal:
Dev Comp Immunol
Year:
2008
Document type:
Article
Affiliation country:
Brasil