[Variation of TFAR-19 protein expression in the thymus of emotional stress mice and the regulatory effect of Modified Xiaoyao Pill].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 27(7): 626-8, 2007 Jul.
Article
in Zh
| MEDLINE
| ID: mdl-17717924
ABSTRACT
OBJECTIVE:
To study the variation of TFAR-19 protein expression and Annexin-V apoptosis protein proportion in the mice thymus cell apoptosis procedure induced by water platform environment or electric emotional stress and the regulatory effect of Modified Xiaoyao Pill (MXP).METHODS:
The mouse emotional stress model was established by water platform equipment or electrical stimulation. The serum glucocorticoid was detected by radioimmunoassay, the TFAR-19 protein was detected by flow cytometry analysis, and Annexin-V apoptosis protein proportion was calculated by immunofluorescence technique.RESULTS:
In the groups of mouse stressed by water platform environment, the level of serum glucocorticoid, the TFAR-19 protein expression and the Annexin-V apoptosis protein proportion increased in the thymus cell along with the stress time prolonging (P<0.05 or P <0.01). The serum glucocorticoid level in mice treated with MXP was lower than that in the untreated group (P <0.05). In the groups of emotional stressed mouse established by electrical stimulation, the above-mentioned variations also revealed. All these variations could be alleviated with MXP (P<0.05).CONCLUSION:
The water platform environment stress is a chronic continuous stress and electrical stimulation is an acute smooth stress, both of them could damage thymus function through neuro-endocrineo-immune network, but different in duration for causing severe injury. Chinese medicine MXP can alleviate the damage of thymus induced by either of them to certain degree.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stress, Psychological
/
Thymus Gland
/
Drugs, Chinese Herbal
/
Apoptosis Regulatory Proteins
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2007
Document type:
Article