Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration.
Biochem Biophys Res Commun
; 365(2): 285-90, 2008 Jan 11.
Article
in En
| MEDLINE
| ID: mdl-17986384
ABSTRACT
Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. Wnt signalling is mediated through cell-cell interaction and is involved in many developmental processes and cellular functions. In this study, we investigated the possible function of Wnt5a and the non-canonical Wnt pathway in human endothelial cells. We found that Wnt5a-mediated non-canonical Wnt signalling regulated endothelial cell proliferation. Blocking this pathway using antibody, siRNA or a down-stream inhibitor led to suppression of endothelial cell proliferation, migration, and monolayer wound closure. We also found that the mRNA level of Wnt5a is up-regulated when endothelial cells are treated with a cocktail of inflammatory cytokines. Our findings suggest non-canonical Wnt signalling plays a role in regulating endothelial cell growth and possibly in angiogenesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Signal Transduction
/
Proto-Oncogene Proteins
/
Endothelial Cells
/
Wnt Proteins
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2008
Document type:
Article
Affiliation country:
Reino Unido
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA