An efficient method to express GPI-anchor proteins in insect cells.
Biochem Biophys Res Commun
; 365(4): 657-63, 2008 Jan 25.
Article
in En
| MEDLINE
| ID: mdl-18029261
ABSTRACT
Glycosylphosphatidylinositols (GPIs) constitute a class of glycolipids that have various functions, the most basic being to attach proteins to the surface of eukaryotic cells. GPIs have to be taken into account, when expressing surface antigens from parasitic protozoa in heterologous systems. The synthesis of the GPI-anchors was previously reported to be drastically decreased to almost background level following baculovirus infection. Here we describe a new method to express GPI-anchor proteins in insect cells relying on using of a supplementary baculovirus construct that overexpresses the N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the enzyme catalyzing the second step in the GPI biosynthetic pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Engineering
/
Baculoviridae
/
Glycosylphosphatidylinositols
/
Spodoptera
/
Amidohydrolases
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2008
Document type:
Article
Affiliation country:
Alemania