Optical fingerprinting of peptides using two-dimensional infrared spectroscopy: proof of principle.
Anal Biochem
; 374(2): 358-65, 2008 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-18062912
ABSTRACT
We employ a particular form of two-dimensional infrared four-wave mixing (2DIR FWM) as a vibrational spectroscopic tool to quantify the amino acid content of a number of peptides. Vibrational features corresponding to ring modes of the aromatic groups of phenylalanine (Phe) and tyrosine (Tyr), as well as a methylene mode that is used as an internal reference, are identified. We show that the ratios of the integrated intensities, and the amplitudes, of the aromatic peaks of Phe and Tyr relative to the methylene integrated intensity, and amplitude, are proportional to the actual ratio of Phe and Tyr to CH(2) in the samples within a precision of +/-12.5%. This precision is shown to be sufficient to use this form of 2DIR spectroscopy as a possible proteins fingerprinting tool.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrophotometry, Infrared
/
Peptide Mapping
/
Optics and Photonics
Type of study:
Diagnostic_studies
Language:
En
Journal:
Anal Biochem
Year:
2008
Document type:
Article
Affiliation country:
Reino Unido