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Fusion of circulating blood cells with solid-organ tissue cells in clinical stem cell transplants: a potential therapeutic model?
Körbling, Martin; de Lima, Marcos J; Thomas, Elizabeth; Khanna, Abha; Najjar, Amer M; Gu, Jun; Gelovani, Juri G; Broaddus, Russell.
Affiliation
  • Körbling M; University of Texas, Department of Stem Cell Transplantation and Cellular Therapy, Unit 423, M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA. mkorblin@mdanderson.org
Regen Med ; 3(2): 157-64, 2008 Mar.
Article in En | MEDLINE | ID: mdl-18307399
AIM: In female patients who have undergone sex-mismatched peripheral blood stem cell transplantation, solid-organ tissue cells have been identified that carry the Y-chromosome. How genetic material from circulating cells is acquired by solid-organ tissue cells is debated. The purpose of this study was to provide clinical evidence for cell fusion between circulating cells and solid-organ tissue cells. MATERIAL & METHODS: The clinical model was a male-into-female blood stem cell transplantation setting using the Y-chromosome as a blood-derived cell marker and the patient's BCR/ABL fusion gene. Endometrial cells were chosen as the target cells because of their uniquely female genotype. RESULTS: The Y-chromosome and the BCR/ABL fusion gene were identified by fluorescence in situ hybridization and were colocalized with estrogen receptor-staining endometrial cells. Both donor-derived Y-chromosome and patient-derived fusion gene were identified in the same endometrial cells, thereby indicating cell fusion as the mechanism for genetic material transfer in a clinical setting. CONCLUSION: These findings contribute to our understanding of how blood-derived cells interact with solid-organ tissue cells.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Cell Fusion / Stem Cell Transplantation / Models, Biological Type of study: Prognostic_studies Limits: Adult / Female / Humans / Male Language: En Journal: Regen Med Year: 2008 Document type: Article Affiliation country: Estados Unidos Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Cell Fusion / Stem Cell Transplantation / Models, Biological Type of study: Prognostic_studies Limits: Adult / Female / Humans / Male Language: En Journal: Regen Med Year: 2008 Document type: Article Affiliation country: Estados Unidos Country of publication: Reino Unido