Low-voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells.
Microsc Res Tech
; 71(9): 659-62, 2008 Sep.
Article
in En
| MEDLINE
| ID: mdl-18512738
ABSTRACT
Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane-bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low-voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pancreas
/
Simian virus 40
/
Cytoplasmic Granules
/
Microscopy, Electron, Transmission
Limits:
Animals
Language:
En
Journal:
Microsc Res Tech
Journal subject:
DIAGNOSTICO POR IMAGEM
Year:
2008
Document type:
Article
Affiliation country:
Canadá