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Mammalian cells loaded with platinum-containing molecules are sensitized to fast atomic ions.
Usami, N; Furusawa, Y; Kobayashi, K; Lacombe, S; Reynaud-Angelin, A; Sage, E; Wu, Ting-Di; Croisy, A; Guerquin-Kern, J-L; Le Sech, C.
Affiliation
  • Usami N; Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Oho, Tsukuba, Japan.
Int J Radiat Biol ; 84(7): 603-11, 2008 Jul.
Article in En | MEDLINE | ID: mdl-18661376
ABSTRACT

PURPOSE:

This work investigates whether a synergy in cell death induction exists in combining atomic ions irradiation and addition of platinum salts. Such a synergy could be of interest in view of new cancer therapy protocol based on atomic ions--hadrontherapy--with the addition of radiosensitizing agents containing high-Z atoms. The experiment consists in irradiating by fast ions cultured cells previously exposed to dichloroterpyridine Platinum (PtTC) and analyzing cell survival by a colony-forming assay. MATERIALS AND

METHODS:

Chinese Hamster Ovary (CHO) cells were incubated for six hours in medium containing 350 microM PtTC, and then irradiated by fast ions C(6+) and He(2+), with Linear Energy Transfer (LET) within range 2-70 keV/microm. In some experiments, dimethyl sulfoxide (DMSO) was added to investigate the role of free radicals. The intracellular localization of platinum was determined by Nano Secondary Ion Mass Spectroscopy (Nano-SIMS).

RESULTS:

For all LET examined, cell death rate is largely enhanced when irradiating in presence of PtTC. At fixed irradiation dose, cell death rate increases with increasing LET, while the platinum relative effect is larger at low LET.

CONCLUSION:

This finding suggests that hadrontherapy or protontherapy therapeutic index could be improved by combining irradiation procedure with concomitant chemotherapy protocols using platinum salts.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoplatinum Compounds / Carbon / Linear Energy Transfer / Heavy Ions / Helium Type of study: Guideline Limits: Animals Language: En Journal: Int J Radiat Biol Journal subject: RADIOLOGIA Year: 2008 Document type: Article Affiliation country: Japón

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoplatinum Compounds / Carbon / Linear Energy Transfer / Heavy Ions / Helium Type of study: Guideline Limits: Animals Language: En Journal: Int J Radiat Biol Journal subject: RADIOLOGIA Year: 2008 Document type: Article Affiliation country: Japón