Performance characteristics of the platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid.
Clin Vaccine Immunol
; 15(12): 1760-3, 2008 Dec.
Article
in En
| MEDLINE
| ID: mdl-18845830
ABSTRACT
We have evaluated the Platelia Aspergillus enzyme immunoassay for detection of galactomannan in bronchoalveolar lavage (BAL) specimens in solid organ transplant patients with aspergillosis. The precision and reproducibility in serum or BAL to which galactomannan was added were similar. Sensitivity was 81.8% in patients with aspergillosis, and specificity was 95.8% in lung transplant patients who underwent BAL for surveillance for infection or rejection. Among transplant controls, positive results were more common in patients (i) who underwent diagnostic BAL performed for evaluation of symptoms or chest computed tomographic abnormalities, (ii) who had undergone lung transplantation, or (iii) who were colonized with Aspergillus. Galactomannan testing in BAL is useful for diagnosis of aspergillosis in transplant patients. The significance of positive results in patients without confirmed aspergillosis requires further evaluation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aspergillosis
/
Aspergillus
/
Bronchoalveolar Lavage Fluid
/
Immunoenzyme Techniques
/
Mannans
/
Antigens, Fungal
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
Clin Vaccine Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2008
Document type:
Article
Affiliation country:
Estados Unidos