Direct application of the PCR restriction analysis method for identifying NTM species in AFB smear-positive respiratory specimens.
Int J Tuberc Lung Dis
; 12(11): 1344-6, 2008 Nov.
Article
in En
| MEDLINE
| ID: mdl-18926049
ABSTRACT
The early differentiation of Mycobacterium tuberculosis from non-tuberculous mycobacteria (NTM) and the identification of NTM species are crucial for the proper management of patients with smear-positive sputum. We evaluated the usefulness of a polymerase chain reaction restriction analysis (PRA) method based on the rpoB gene for identifying NTM species in a study of 121 smear-positive respiratory specimens with presumed NTM. The PRA method amplified mycobacterial DNA in 72 specimens (60%) and differentiated NTM species correctly in 68 (94%). The PRA method could be a useful and rapid method for identifying NTM species in smear-positive respiratory specimens when urgent clinical decisions are required.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Lung Diseases
/
Mycobacterium Infections
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Int J Tuberc Lung Dis
Year:
2008
Document type:
Article