Growth arrest of Synechocystis sp. PCC6803 by superoxide generated from heterologously expressed Rhodobacter sphaeroides chlorophyllide a reductase.
FEBS Lett
; 583(1): 219-23, 2009 Jan 05.
Article
in En
| MEDLINE
| ID: mdl-19084011
The photosynthetic growth of Synechocystis sp. PCC6803 ceased upon expression of Rhodobacter sphaeroides chlorophyllide a reductase (COR). However, an increase in cytosolic superoxide dismutase level in the recombinant Synechocystis sp. PCC6803 completely reversed the growth cessation. This demonstrates that COR generates superoxide in Synechocystis sp. PCC6803. Considering the dissolved oxygen (DO) level suitable for COR, the intracellular DO of this oxygenic photosynthetic cell appears to be low enough to support COR-mediated superoxide generation. The growth arrest of Synechocystis sp. PCC6803 by COR may give an insight into the evolutionary path from bacteriochlorophyll a biosynthetic pathway to chlorophyll a, which bypasses COR reaction.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rhodobacter sphaeroides
/
Superoxides
/
Evolution, Molecular
/
Oxidoreductases Acting on CH-CH Group Donors
/
Synechocystis
Language:
En
Journal:
FEBS Lett
Year:
2009
Document type:
Article
Country of publication:
Reino Unido