Prevalence of Trichinella spp. in North Spain wild fauna and new variety of Trichinella britovi identification.
Vet Parasitol
; 159(3-4): 222-4, 2009 Feb 23.
Article
in En
| MEDLINE
| ID: mdl-19084339
ABSTRACT
Trichinella spiralis and Trichinella britovi live in apparent sympatry among wild fauna of the Iberian Peninsula. In the present study 105 Trichinella isolates from wild mammals were typed by inter-sequence simple repeat PCR (ISSR-PCR). All isolates identified as T. spiralis were indistinguishable from the ISS48 reference strain. Among those belonging to T. britovi, four variations were clearly distinguishable; two of them, ISS11 C-76 and ISS86 MON, had been previously detected while the ISS2 reference strain and Trichinella Rioja 3, (MVUL/SP/02/R3) had not been reported before. The newly distinguished genotype of T. britovi was analyzed by ISSR-PCR, multiplex-PCR, UARR sequencing, and single larva cross-breeding with the other T. britovi genotypes including Trichinella T8 (ISS49). Among all of them, the ISS11 and ISS2 isolates were found to be the most frequent. The uniformity found within T. spiralis isolates is consistent with its recent introduction in Iberian Peninsula, whereas the presence of four variations within T. britovi suggests that this species is an endemic species. Orographical diversity of the West-End of Eurasian Region could act to preserve population diversity observed within T. britovi.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trichinella
/
Trichinellosis
/
Animals, Wild
Type of study:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Vet Parasitol
Year:
2009
Document type:
Article
Affiliation country:
España