[Intracellular clearance of Coxsakievirus B3 infection by short interfering RNA and its mechanism study].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 22(4): 260-2, 2008 Aug.
Article
in Zh
| MEDLINE
| ID: mdl-19105337
ABSTRACT
OBJECTIVE:
To evaluate the possibility of short interfering RNA (siRNA) inhibiting Coxsackievirus B3 (CVB3) infection in vitro, and discover the mechanism initially.METHODS:
We obtained proper effective dosage of siRNA by observing cytopathic effect (CPE). Estimate its antiviral activities and its pathway of siRNA by Western Blot assay and RT-PCR.RESULTS:
Results showed that siRNA-3753 can be effectively transfected into HeLa cells, we can achieve a high transfection efficiency up to 98.77% and its effect can last for 48 h stably in cells. 0.6 micromol/L siRNA-3753 got a high inhibiting effect of virus and didn't show any toxicity to cells. So we consider this concentration as the experimental concentration. siRNA-3753 can debase virus reproduction. The antiviral effect is sequence-specific and is not attributable to either interferon or the interferon response effectors protein kinase R (PKR).CONCLUSION:
The data confirmed that siRNA can effectively inhibit CVB3 infection in vitro, its antivirus effect was gained from specific debase of virus genome.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enterovirus B, Human
/
Coxsackievirus Infections
/
RNA, Small Interfering
/
RNA Interference
Limits:
Humans
Language:
Zh
Journal:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Journal subject:
VIROLOGIA
Year:
2008
Document type:
Article
Affiliation country:
China