Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Mol Biother
; 3(2): 95-102, 1991 Jun.
Article
in En
| MEDLINE
| ID: mdl-1910625
ABSTRACT
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific cytotoxicity of CTL were achieved in 4 to 6 weeks of culture. The predominant populations in cultured LNC-derived CTL were CD2+, CD3+, CD4-, CD8+, CD56-, and HLA-DR+ T cells. These data suggested that tumor-involved LNC may provide an alternative source for the generation of tumor-specific CTL in adoptive immunotherapy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes, Cytotoxic
/
Lymphocytes, Tumor-Infiltrating
/
Interleukin-2
/
Lymph Nodes
/
Melanoma
Limits:
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Mol Biother
Journal subject:
ALERGIA E IMUNOLOGIA
/
NEOPLASIAS
/
TERAPEUTICA
Year:
1991
Document type:
Article
Affiliation country:
Canadá