Protective effect of polygoni cuspidati radix and emodin on Vibrio vulnificus cytotoxicity and infection.
J Microbiol
; 46(6): 737-43, 2008 Dec.
Article
in En
| MEDLINE
| ID: mdl-19107405
ABSTRACT
Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vibrio Infections
/
Plant Extracts
/
Emodin
/
Vibrio vulnificus
/
Fallopia japonica
Limits:
Animals
/
Humans
Language:
En
Journal:
J Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2008
Document type:
Article