Molecular ecology of a facultative swine waste lagoon.
Lett Appl Microbiol
; 48(4): 486-92, 2009 Apr.
Article
in En
| MEDLINE
| ID: mdl-19243502
AIMS: To investigate the microbial ecology of three facultative swine waste lagoons. METHODS AND RESULTS: Phylogenetic analysis of sequences in a 16S rRNA gene clone library and fluorescence in situ hybridization (FISH) analyses were used to assess bacterial diversity in a swine waste lagoon. FISH analysis and Gram-staining were used to compare the microbial communities of all three swine waste lagoons. Six operational taxonomic units were in high relative abundance and corresponded to the following phylotypes; Thiolamprovum, Verrucomicrobia, Acholeplasma, Turicibacter, Clostridium and Bacteroides. PCR was employed to detect the genes apsA and dsrAB which encode for enzymes specifically associated with dissimilatory sulfate-reduction within sulfate-reducing bacteria (SRB). Amplification of these genes confirmed their presence within the lagoons. CONCLUSIONS: All lagoons were dominated by purple sulfur bacteria, affiliated to Thiolamprovum pedioforme. The molecular identification of fermentative bacteria and SRB indicate the following metabolic processes within such facultative ponds: sulfur-cycling, fermentation, inter-species hydrogen transfer and carbon cycling. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides the first molecular evidence for the existence of a sulfur cycle which is linked to phototrophic sulfide oxidation by purple bacteria and organotrophic sulfate-reduction by SRB.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfur
/
Swine
/
Bacteria
/
Water Microbiology
/
Waste Disposal, Fluid
/
Ecosystem
Limits:
Animals
Language:
En
Journal:
Lett Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
Australia
Country of publication:
Reino Unido