Lipopolysaccharide-induced early response genes in bovine peripheral blood mononuclear cells implicate GLG1/E-selectin as a key ligand-receptor interaction.

ABSTRACT

This study uses integrated global gene expression information and knowledge of the regulatory events in cells to identify transcription networks controlling peripheral blood mononuclear cells' (PBMCs) immune response to lipopolysaccharide (LPS) and to identify the molecular and cellular pathways' responses to LPS. We identified that 464 genes, including at least 17 transcription factors, are significantly induced by 2-h LPS stimulation using a high-density bovine microarray platform at a very stringent false discovery rate = 0%. The networks show that, in the LPS-stimulated PBMCs, altered gene expression was transcriptionally regulated via those transcription factors through potential interaction within the pathway networks. Functional analyses revealed that LPS induces unique pathways, molecular functions, biological processes, and gene networks. In particular, gene expression data identified Golgi complex-localized glycoprotein 1/endothelial-selectin as a key ligand-receptor interaction in the early response of cells.