A quantitative method for analyzing establishing-efficiency of persistent viral infection.
Microbiol Immunol
; 53(5): 259-65, 2009 May.
Article
in En
| MEDLINE
| ID: mdl-19457166
ABSTRACT
A quantitative method for analyzing establishing-efficiency of persistent infection was devised. The efficiency of hPIV2 CA and SV5 T1 strains was found to be high, that is, 0.1 approximately 0.3 (an efficiency of 1.0 indicates that 100% of the virus-infected cells became persistently infected). The efficiency of the SV5 WR strain was also high, approximately 0.1, though the virus had no ability to immediately establish a steady state of persistent infection in whole cell-culture systems. At about 0.0007, the efficiency of SV41 was almost the same as that of the hPIV2 Toshiba strain. The establishing efficiencies of various rSeV were further analyzed in detail. The efficiencies of the rSeV(PA), rSeV(Ppi) and rSeV(HNpi) were below the limit of detection, while that of rSeV(Lpi) was nearly 1. Although the efficiency was around 0.001, the rSeV(Mpi) and the rSeV(Fpi) were unexpectedly found to be capable of forming persistently-infected cells, indicating that both the Fpi and Mpi proteins contribute to the establishing efficiency of persistent infection of SeVpi.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Cultivation
/
Paramyxovirinae
/
Paramyxoviridae Infections
Type of study:
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Microbiol Immunol
Year:
2009
Document type:
Article
Affiliation country:
Japón