Determination of octopine by pre-column fluorescence derivatization using benzoin.
Biochem Int
; 23(6): 1035-9, 1991 Apr.
Article
in En
| MEDLINE
| ID: mdl-1953798
ABSTRACT
A sensitive fluorimetric method for the determination of octopine, a member of opine family, is presented. The method is based on the formation of a fluorescent derivative of octopine with benzoin and the separation by high performance liquid chromatography using a reversed-phase column (Kaseisorb LC ODS-300) within 20 min. The octopine derivative is completely separated from other guanidino compounds including arginine which is generally very high in marine invertebrates. This method gives higher sensitivity, 5 pmol minimum detection, and better reproducibility than the electrophoresis method and colorimetric method.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginine
/
Fluorometry
Limits:
Animals
Language:
En
Journal:
Biochem Int
Year:
1991
Document type:
Article
Affiliation country:
Japón