Lowered albumin extravasation rate in heart but not in other organs in beta3-integrin-deficient mice.
Acta Physiol (Oxf)
; 197(4): 305-11, 2009 Dec.
Article
in En
| MEDLINE
| ID: mdl-19645751
ABSTRACT
AIM:
The vascular protein permeability is dependent on the integrity of the vascular wall. The heart capillaries in male mice lacking beta3 integrins have an immature phenotype. Previously, we have demonstrated a role for alphavbeta3 integrins in control of interstitial fluid pressure (Pif) and thereby in the fluid flux during inflammation. We wanted to explore a possible role for alphavbeta3 integrins in controlling capillary protein permeability during control situation and inflammation.METHODS:
We performed double-tracer and microdialysis experiments on beta3-integrin-deficient mice and wild type control mice. We also measured blood pressure and heart rate in the two mice strains.RESULTS:
We found reduced albumin extravasation (during 25 min) in the heart capillaries (0.053 +/- 0.003 vs. 0.087 +/- 0.009 mL g(-1) dw, P < 0.05), and an increased cardiac mass/body weight (5.3 x 10(-3) +/- 0.3 x 10(-3) vs. 3.8 x 10(-3) +/- 0.1 x 10(-3), P < 0.01) in the beta3-integrin-deficient mice (n = 6) compared with the controls (n = 6). Heart rate and blood pressure were the same in mice with and without beta3-integrins. No difference in permeability was found in other tissues studied, or under local inflammation.CONCLUSION:
These results show a function for the alphavbeta3 integrin in the regulation of protein permeability, selective for the heart capillaries.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Capillary Permeability
/
Extracellular Fluid
/
Albumins
/
Heart
/
Myocardium
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Acta Physiol (Oxf)
Journal subject:
FISIOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
Noruega