Cholesterol-loaded-cyclodextrins and fertility potential of stallions spermatozoa.
Anim Reprod Sci
; 118(2-4): 255-64, 2010 Apr.
Article
in En
| MEDLINE
| ID: mdl-19762177
ABSTRACT
Irreversible damage occurs to spermatozoal membranes, during the phase transition, when spermatozoa are cooled from room temperature to 5 degrees C. Some of this damage can be ameliorated by adding cholesterol to the membrane, thereby altering membrane lipid composition. Adding cholesterol-loaded cyclodextrins (CLCs) to stallion spermatozoa prior to freezing, increases cell cryosurvival. However, the fertilizing potential of CLC-treated stallion spermatozoa is unknown. To address this, experiments were conducted which evaluated the ability of CLC-treated stallion spermatozoa to capacitate, acrosome react, and bind to the zona pellucida in vitro, and to fertilize oocytes in vivo. When CLC-treated cryopreserved stallion spermatozoa were treated with various agents to induce capacitation and the acrosome reaction (AR), dilauroylphosphatidylcholine (PC-12) and lysophosphatidylcholine (LPC) induced the AR in control cells (62% and 55%, respectively) but not in CLC-treated cells (17% and 14%, respectively, P<0.05). However, the calcium ionophore A23187 induced the AR in both control- and CLC-treated cells equally well (39%, P>0.05). Control- and CLC-treated stallion spermatozoa bound to ZP of cattle oocytes equally well (0.44+/-0.16 vs. 0.25+/-0.09, respectively; P>0.05). In addition, the fertility rates of mares inseminated with control- and CLC-treated sperm were similar (P>0.05).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Cholesterol
/
Cyclodextrins
/
Fertility
/
Horses
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Anim Reprod Sci
Year:
2010
Document type:
Article
Affiliation country:
Estados Unidos