[Study on proteomics of Jurkat cells treated with the extracts from Prunella vulgaris].
Zhong Yao Cai
; 32(6): 917-22, 2009 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-19764330
ABSTRACT
OBJECTIVE:
To analyse the differential proteins of Jurkat cells after treated with the extracts from Prunella vulgaris using two-dimensional electrophoresis and mass spectrum.METHODS:
Jurkat cell growth inhibitive effect of the extracts from Prunella vulgaris was analyzed by MTT assay. The total proteins of the cells were extracted after treated with the extracts in a dose of 20 microg/mL. Then 2D and MALDI-TOF-MS were used to assess the differential proteins.RESULTS:
The extracts from Prunella vulgaris could depress the proliferation of Jurkat cells in a dose-dependent manner. After 2-DE and MALDI-TOF-MS,11 proteins were identified successfully, including glyceraldehyde-3-phosphate dehydrogenase, coagulation factor VII, Heterogeneous nuclear ribonucleoprotein L, heat shock 70 kDa protein 8 isoform 2, immunoglobulin heavy chain variable region, heterogeneous nuclear ribonucleoprotein A2/B1, zinc finger protein 43, chaperonin containing TCP1, subunit 6A (zeta 1), isoform CRA_b, etc.CONCLUSION:
The extracts from Prunella vulgaris could inhibit the growth of Jurkat cells significantly, and lead the proteomics change of Jurkat cells, which may be related to the anti-tumor effect of the extracts from Prunella vulgaris.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drugs, Chinese Herbal
/
Prunella
/
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
/
Proteomics
/
HSC70 Heat-Shock Proteins
/
Antineoplastic Agents, Phytogenic
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Zhong Yao Cai
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2009
Document type:
Article
Affiliation country:
China