[Effects of recombinant soluble MICA protein on the biologic activities of NK cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 25(10): 903-6, 2009 Oct.
Article
in Zh
| MEDLINE
| ID: mdl-19811738
ABSTRACT
AIM:
To study the effects of recombinant soluble MHC class I chain-related protein A (sMICA) on the cytotoxicity, secretion of IFN-gamma, proliferation and apoptosis of peripheral NK cells.METHODS:
After NK cells were co-cultured with recombinant soluble MICA proteins overnight, the cytotoxicity of NK cell on target cells was detected by flow cytometry. The supernant was collected to determine the concentration of IFN-gamma by ELISA. The proliferation of NK cells to sMICA was detected by MTS/PMS. NK cells were labeled with annexin V and PI to analyze their apoptosis.RESULTS:
Soluble MICA inhibited the cytotoxicity of NK cells and down-regulated the secretion of IFN-gamma, but it showed no effects on the proliferation and apoptosis of freshly isolated peripheral NK cells.CONCLUSION:
The soluble MICA shedding from tumor cells could be a pathway of cancer immune evasion by down-regulating the biologic activities of NK cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Killer Cells, Natural
/
Histocompatibility Antigens Class I
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
China