Prolonged cigarette smoke exposure decreases heme oxygenase-1 and alters Nrf2 and Bach1 expression in human macrophages: roles of the MAP kinases ERK(1/2) and JNK.
FEBS Lett
; 583(21): 3508-18, 2009 Nov 03.
Article
in En
| MEDLINE
| ID: mdl-19822148
ABSTRACT
Tobacco may be involved in the decreased macrophage heme oxygenase-1 (HO-1) expression described in smoking-induced severe emphysema, via the nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1)-BTB and CNC homology 1, basic leucine zipper transcription factor 1 (Bach1) pathway. We assessed in vitro effects of cigarette smoke condensate (CS) in the human monocyte/macrophage cell line (THP-1). CS exposure led to increased HO-1 and nuclear Nrf2 expression (6 h) followed by decreased HO-1 expression concomitantly with nuclear Nrf2/Bach1 ratio decrease (72h). CS-induced mitogen-activated protein kinase (MAPK) phosphorylation. Extracellular-signal-regulated kinase(1/2) (ERK(1/2)) and c-Jun NH2-terminal kinase (JNK) inhibition completely abrogated CS effects on HO-1 expression and nuclear Nrf2/Bach1 translocation. These results suggest that ERK(1/2) and JNK are involved in CS-induced biphasic HO-1 expression by a specific regulation of Nrf2/Keap1-Bach1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Smoking
/
Gene Expression Regulation
/
Mitogen-Activated Protein Kinases
/
Heme Oxygenase-1
/
Basic-Leucine Zipper Transcription Factors
/
NF-E2-Related Factor 2
/
Fanconi Anemia Complementation Group Proteins
/
Macrophages
Type of study:
Etiology_studies
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2009
Document type:
Article
Affiliation country:
Francia