Proteome analysis reveals candidate markers of disease progression in amyotrophic lateral sclerosis (ALS).
Neurosci Lett
; 468(1): 23-7, 2010 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-19853641
ABSTRACT
OBJECTIVES:
In amyotrophic lateral sclerosis (ALS) the pathological determinants of disease progression remain poorly understood. We aimed to identify a characteristic CSF protein pattern that could provide new candidate biomarkers of disease progression in ALS.METHODS:
Using the two-dimensional difference in gel electrophoresis (2-D-DIGE), we compared CSF samples from patients with ALS that showed a rapid progression of disease (ALS-rp, n=9) over a follow-up time of 2 years and from patients with ALS that showed a slow progression of disease over follow-up (ALS-sl, n=9) over the same period. Protein spots that showed significant differences between patients and controls were selected for further analysis by MALDI-TOF mass spectrometry. For validation of identified spots ELISA and nephelometry were performed for two candidate proteins on a second cohort of patients (n=40).RESULTS:
We identified 6 different proteins and their isoforms which were all upregulated in ALS-rp as compared to ALS-sl (heat shock protein1, alpha-1 antitrypsin, fetuin-A precursor, transferrin, transthyretin (TTR), nebulin-related anchoring protein). For Fetuin-A and TTR, our findings could be confirmed by quantitative assay.CONCLUSIONS:
Fetuin-A and TTR are promising candidate markers for disease progression in ALS that warrant further evaluation on a larger cohort of patients.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteome
/
Amyotrophic Lateral Sclerosis
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Aged
/
Humans
/
Middle aged
Language:
En
Journal:
Neurosci Lett
Year:
2010
Document type:
Article
Affiliation country:
Alemania