Automated imaging MS: Toward high throughput imaging mass spectrometry.
J Proteomics
; 73(6): 1279-82, 2010 Apr 18.
Article
in En
| MEDLINE
| ID: mdl-19896567
ABSTRACT
The term molecular histology has been used to convey the potential of imaging mass spectrometry to describe tissue by its constituent peptides and proteins, and to link this with established histological features. The low throughput of imaging mass spectrometry has been one of the factors inhibiting a full investigation of the clinical potential of molecular histology. Here we report the development of an automated set-up, consisting of a controlled environment sample storage chamber, a sample loading robot, and a MALDI-TOF/TOF mass spectrometer, all controlled by a single user interface. The automated set-up is demonstrated to have the positional stability and experimental reproducibility necessary for its clinical application.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteomics
Limits:
Humans
Language:
En
Journal:
J Proteomics
Journal subject:
BIOQUIMICA
Year:
2010
Document type:
Article
Affiliation country:
Países Bajos