The role of the substrate lipid in processive glycan polymerization by the peptidoglycan glycosyltransferases.
J Am Chem Soc
; 132(1): 48-9, 2010 Jan 13.
Article
in En
| MEDLINE
| ID: mdl-20017480
ABSTRACT
The peptidoglycan glycosyltransferases (PGTs) catalyze the processive polymerization of a C55 lipid-linked disaccharide (Lipid II) to form peptidoglycan, the main component of the bacterial cell wall. Our ability to understand this reaction has been limited due to challenges identifying the appropriate substrate analogues to selectively interrogate the donor (the elongating strand) and acceptor (Lipid II) sites. To address this problem, we have developed an assay using synthetic substrates that can discriminate between the donor and acceptor sites of the PGTs. We have shown that each site has a distinct lipid length preference. We have also established that processive polymerization depends on the length of the lipid attached to the donor.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymers
/
Polysaccharides
/
Peptidoglycan Glycosyltransferase
/
Lipid Metabolism
Language:
En
Journal:
J Am Chem Soc
Year:
2010
Document type:
Article
Affiliation country:
Estados Unidos