An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea.
Science
; 252(5002): 114-7, 1991 Apr 05.
Article
in En
| MEDLINE
| ID: mdl-2011746
ABSTRACT
Derivatives of erythromycin with modifications at their C-6 position are generally sought for their increased stability at acid pH, which in turn may confer improved pharmacological properties. A recombinant mutant of the erythromycin-producing bacterium, Saccharopolyspora erythraea, produced an erythromycin derivative, 6-deoxyerythromycin A, that could not be obtained readily by chemical synthesis. This product resulted from targeted disruption of the gene, designated eryF (systematic nomenclature, CYP107), that apparently codes for the cytochrome P450, 6-deoxyerythronolide B (DEB) hydroxylase, which converts DEB to erythronolide B (EB). Enzymes normally acting on EB can process the alternative substrate DEB to form the biologically active erythromycin derivative lacking the C-6 hydroxyl group.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Erythromycin
/
Cytochrome P-450 Enzyme System
/
Genes, Bacterial
/
Mixed Function Oxygenases
Language:
En
Journal:
Science
Year:
1991
Document type:
Article