The proteome complement of Nicotiana tabacum Bright-Yellow-2 culture cells.
Proteomics
; 10(13): 2545-50, 2010 Jul.
Article
in En
| MEDLINE
| ID: mdl-20405476
The Nicotiana tabacum Bright-Yellow-2 (BY2) cell line is one of most commonly used plant suspension cell lines and offers interesting properties, such as fast growth, amenability to genetic transformation, and synchronization of cell division. To build a proteome reference map of BY2 cell proteins, we isolated the soluble proteins from N. tabacum BY2 cells at the end of the exponential growth phase and analyzed them by 2-DE and MALDI TOF-TOF. Of the 1422 spots isolated, 795 were identified with a significant score, corresponding to 532 distinct proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Nicotiana
/
Proteome
Type of study:
Prognostic_studies
Language:
En
Journal:
Proteomics
Journal subject:
BIOQUIMICA
Year:
2010
Document type:
Article
Affiliation country:
Bélgica
Country of publication:
Alemania