Is yessotoxin the main phycotoxin in Croatian waters?
Mar Drugs
; 8(3): 460-70, 2010 Mar 05.
Article
in En
| MEDLINE
| ID: mdl-20411109
ABSTRACT
With the aim of investigating whether yessotoxin (YTX) is responsible for diarrhetic shellfish poisoning (DSP) events in Croatian waters, three different methods were combined a modified mouse bioassay (MBA) that discriminates YTX from other DSP toxins, the enzyme-linked immunosorbent assay method (ELISA) and liquid chromatography-mass spectrometry (LC-MS/MS). Among 453 samples of mussels and seawater analyzed in 2007, 10 samples were DSP positive. Results obtained by the modified MBA method revealed that most of the samples were positive for YTX, with the exception of samples from Lim Bay (LB 1) The ELISA method also identified the presence of YTX in these samples. DSP toxin profiles showed the presence of okadaic acid (OA) in three, and YTX in four out of nine samples that were analyzed by LC-MS/MS. The phytoplankton community structure pattern revealed Lingulodinium polyedrum (Stein) Dodge, which was present in the water prior to and/or during toxicity events at low concentrations (80 to 1440 cells L(-1)), as a potential YTX producing species. It is proposed that L. polyedrum cells accumulated in mussels and the subsequently observed toxicity may be related to metabolism after ingestion, resulting in carboxy YTX as the major analog in the mussel.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seawater
/
Environmental Monitoring
/
Bivalvia
/
Oxocins
/
Marine Toxins
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Mar Drugs
Journal subject:
BIOLOGIA
/
FARMACOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Croacia
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND