Accurate analysis of urea in milk and milk powder by isotope dilution gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci
; 878(19): 1634-8, 2010 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-20434964
ABSTRACT
A high order method for measuring urea concentrations in milk and milk powder was developed. The method can be applied to certify the concentration of urea in some new milk and milk powder CRMs. This high accurate method for analysis of milk is valuable given the inherent challenges associated with the complexity of the sample matrix. A measurement procedure based on gas chromatography/isotope dilution mass spectrometry (GC/IDMS) was developed. Samples were pre-treated with acetonitrile to remove proteins and the method was applied to determine urea concentrations in milk and milk powder. Excellent precision was obtained, with within- and between-set coefficients of variation of 0.15-0.46 and 0.18-0.65%, respectively. The measurement uncertainty is evaluated. The method can trace to mass.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Urea
/
Milk
/
Isotope Labeling
/
Gas Chromatography-Mass Spectrometry
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2010
Document type:
Article