Maintenance of red blood cell integrity by AMP-activated protein kinase alpha1 catalytic subunit.
FEBS Lett
; 584(16): 3667-71, 2010 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-20670625
ABSTRACT
AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. We previously showed that AMPKalpha1-/- mice develop moderate anemia associated with splenomegaly and high reticulocytosis. Here, we report that splenectomy of AMPKalpha1-/- mice worsened anemia supporting evidence that AMPKalpha1-/- mice developed a compensatory response through extramedullary erythropoiesis in the spleen. Transplantation of bone marrow from AMPKalpha1-/- mice into wild-type recipients recapitulated the hematologic phenotype. Further, AMPKalpha1-/- red blood cells (RBC) showed less deformability in response to shear stress limiting their membrane flexibility. Thus, our results highlight the crucial role of AMPK to preserve RBC integrity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Erythrocyte Deformability
/
AMP-Activated Protein Kinases
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
2010
Document type:
Article
Affiliation country:
Francia