Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method.
Clin Microbiol Infect
; 17(7): 1007-12, 2011 Jul.
Article
in En
| MEDLINE
| ID: mdl-20718803
ABSTRACT
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a fast and reliable technology for the identification of microorganisms with proteomics approaches. Here, we compare an intact cell method and a protein extraction method before application on the MALDI plate for the direct identification of microorganisms in both urine and blood culture samples from clinical microbiology laboratories. The results show that the intact cell method provides excellent results for urine and is a good initial method for blood cultures. The extraction method complements the intact cell method, improving microorganism identification from blood culture. Thus, we consider that MALDI-TOF MS performed directly on urine and blood culture samples, with the protocols that we propose, is a suitable technique for microorganism identification, as compared with the routine methods used in the clinical microbiology laboratory.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Bacteria
/
Bacterial Infections
/
Urine
/
Blood
/
Bacteriological Techniques
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Guideline
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Clin Microbiol Infect
Journal subject:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
España