Development of a sphingosylphosphorylcholine detection system using RNA aptamers.
Molecules
; 15(8): 5742-55, 2010 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-20729797
ABSTRACT
Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphorylcholine
/
Sphingosine
/
Aptamers, Nucleotide
/
SELEX Aptamer Technique
Type of study:
Diagnostic_studies
Language:
En
Journal:
Molecules
Journal subject:
BIOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Japón